期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 82, 期 5, 页码 904-915出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2018.1438169
关键词
Actinomycetes; xylanase; feruloyl esterase; sugarcane bagasse; fermentable sugars
类别
资金
- Japan Science and Technology Agency
- Japan International Cooperation Agency (JST and JICA), Japan
- RONPAKU (Dissertation PhD) Program [R11512]
- Japan Society for the Promotion of Science (JSPS)
- LIPI research grant
The addition of enzymes that are capable of degrading hemicellulose has a potential to reduce the need for commercial enzymes during biomass hydrolysis in the production of fermentable sugars. In this study, a high xylanase producing actinomycete strain (Kitasatospora sp. ID06-480) and the first ethyl ferulate producing actinomycete strain (Nonomuraea sp. ID06-094) were selected from 797 rare actinomycetes, respectively, which were isolated in Indonesia. The addition (30%, v/v) of a crude enzyme supernatant from the selected strains in sugarcane bagasse hydrolysis with low-level loading (1 FPU/g-biomass) of Cellic (R) CTec2 enhanced both the released amount of glucose and reducing sugars. When the reaction with Ctec2 was combined with crude enzymes containing either xylanase or feruloyl esterase, high conversion yield of glucose from cellulose at 60.5% could be achieved after 72h-saccharification.
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