期刊
BIORESOURCE TECHNOLOGY
卷 263, 期 -, 页码 223-231出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.04.078
关键词
Vertical flow constructed wetland; Solid-phase denitrification; Aeration; Functional genes; Nitrogen removal
资金
- National Natural Science Foundation of China [51778007, 51378021]
- National S&T Major Project of China [2012ZX07102-002]
An up-flow vertical flow constructed wetland (AC-VFCW) filled with ceramsite and 5% external carbon source poly(3-hydroxybutyrate-hydroxyvalerate) (PHBV) as substrate was set for nitrogen removal with micro aeration. Simultaneous nitrification and denitrification process was observed with 90.4% NH4+-N and 92.1% TN removal efficiencies. Nitrification and denitrification genes were both preferentially enriched on the surface of PHBV. Nitrogen transformation along the flow direction showed that NH4+-N was oxidized to NO3--N at the lowermost 10 cm of the substrate and NO3--N gradually degraded over the depth. AmoA gene was more enriched at -10 and -50 cm layers. NirS gene was the dominant functional gene at the bottom layer with the abundance of 2.05 x 10(7) copies g(-1) substrate while nosZ gene was predominantly abundant with 7.51 x 10(6) and 2.64 x 10(6) copies g(-1) substrate at the middle and top layer, respectively, indicating that functional division of dominant nitrogen functional genes forms along the flow direction in AC-VFCW.
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