期刊
BIORESOURCE TECHNOLOGY
卷 249, 期 -, 页码 425-430出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.10.049
关键词
Chlorella pyrenoidosa; Immobilized yeast; Co-culture; Sucrose; Biomass
资金
- Natural Science Foundation of Jiangsu Province, China [BK20170495]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [17KJB240003]
- Postdoctoral Science Foundation of China [2016M591933]
Glucose is normally used as the carbon source for heterotrophic cultivation of algal cells, whereas sucrose is difficult to be heterotrophicly utilized by them. In this study, a new co-culture system was developed through mixed culture of Chlorella pyrenoidosa with immobilized Saccharomyces cerevisiae in the dark to effectively obtain pure algal suspension using sucrose as only carbon source. In this system, a pure algal suspension with a concentration of 2.08 g/L was obtained. The lipid content reached 29%, which was higher than that obtained in glucose contained system. In addition, the immobilized yeast beads were repeatedly used for at least three times. Through immobilization, the choice for the yeast strains that are able to hydrolyze sucrose was not limited by its product and pure algal suspension was efficiently obtained. This strategy may effectively decrease the cost of carbon source in the heterotrophic cultivation of microalgae.
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