期刊
BIORESOURCE TECHNOLOGY
卷 263, 期 -, 页码 40-48出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.04.058
关键词
Lignocellulosic biomass; Enzymatic hydrolysis; Pretreatment; Deep eutectic solvent; Lignin valorization; Pretreatment liquor
资金
- University of Missouri Research Board and NMR core facility, United States
In this study, an acidified, aqueous DES comprising choline chloride: glycerol (ChCl: Gly) was used to fractionate switchgrass into three major streams under a relatively mild condition: cellulose-rich pulp, lignin, and xyloserich liquor. The pulp showed good digestibility with about 89% glucose yield. The solvent can be recycled successfully and reused for at least four more pretreatment cycles while maintaining its pretreatment capability. The solvent recycling also improved the lignin recovery from the pretreatment liquor. Lignin recovered from different pretreatment cycles had the beta-O-4 bonds preserved, and shared similar structures with native lignin. Using the pretreatment liquor as a substrate, the oleaginous yeast Rhodotorula toruloides produced 18.7 g/L biomass with lipid and carotenoid titers of 8.1 g/L and 15.0 mg/L, respectively. Overall, this study demonstrated a green process integrating chemical and biological methods toward full utilization of lignocellulosic biomass.
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