期刊
BIORESOURCE TECHNOLOGY
卷 250, 期 -, 页码 828-837出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.09.149
关键词
Silage; Aerobic deterioration; Methane production; Hydrolytic enzyme activity; Microbial community
资金
- Special Fund for Agro-Scientific Research in the Public Interest, China [201503137, 201303080-7]
- National Key Technology Research and Development Program of China [2012BAD14B06]
Ensilage is a commonly used method of preserving energy crops for biogas production. However, aerobic deterioration of silage is an inevitable problem. This study investigated the effect of aerobic deterioration on methane production and microbial community dynamics through anaerobic digestion (AD) of maize stalk silage, following 9 days air exposure of silage. After air exposure, hydrolytic activity and methanogenic archaea amount in AD were reduced, decreasing the specific methane yield (SMY); whereas lignocellulose decomposition during exposure improved the degradability of silage in AD and enhanced SMY, partially compensating the dry matter (DM) loss. 29.3% of the DM and 40.7% of methane yield were lost following 0-9 days exposure. Metagenomic analysis showed a shift from Clostridia to Bacteroidia and Anaerolineae in AD after silage deterioration; Methanosaetaceae was the dominant methanogenic archaea.
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