期刊
BIORESOURCE TECHNOLOGY
卷 267, 期 -, 页码 438-444出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.07.079
关键词
Schizochytrium sp.; Adaptive evolution; Oxidative stress; Antioxidant capacity; Comparative transcriptomic analysis
资金
- Outstanding Youth Foundation of Jiangsu Nature Science Foundation [BK20160092]
- Program for Innovative Research Teams in Universities of Jiangsu Province
- Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture [XTE1829]
Lipid accumulation of Schizochytrium sp. can be induced by stress condition, but this stress-induction usually reduce cell growth and cause oxidative damage, which can eventually lower the lipid yield. Here, adaptive laboratory evolution (ALE) combined high salinity was performed to enhance the antioxidant system and lipid accumulation. The final strain ALE150, which was obtained after 150 days, showed a maximal cell dry weight (CDW) of 134.5 g/L and lipid yield of 80.14 g/L, representing a 32.7 and 53.31% increase over the starting strain, respectively. Moreover, ALE150 exhibited an overall higher total antioxidant capacity (T-AOC) and lower reactive oxygen species (ROS) levels than the starting strain. Furthermore, the regulatory mechanisms responsible for the improved performance of ALE150 were analyzed by transcriptomic analysis. Genes related to the antioxidant enzymes and central carbon metabolism were up-regulation. Moreover, the metabolic fluxes towards the fatty acid synthase (FAS) and polyketide synthase (PKS) pathways were also changed.
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