期刊
BIORESOURCE TECHNOLOGY
卷 250, 期 -, 页码 591-602出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.11.094
关键词
Astaxanthin esters; Fatty acid; Gene expression; Haematococcus pluvialis; LED lighting conditions
资金
- China Scholarship Council
Haematococcus pluvialis is a green microalga of major interest to industry based on its ability to produce large amounts of astaxanthin. Biosynthesis of astaxanthin and its mono-and di-esters was significantly stimulated under 150 mu molm(-2) s(-1) of white LED (W-150) compared with lower light intensities, but the highest astaxanthin amounts were produced under 70 mu molm(-2) s(-1) of blue LED (B-70). Transcripts of astaxanthin biosynthesis genes psy, crtO, and bkt2 were upregulated under W-150, while psy, lcy, crtO, and crtR-B were upregulated by B-70. Total fatty acid content and biosynthesis genes fata and all dgat genes were induced under W-150, while C18:3n6 biosynthesis and dgat2a expression were specifically stimulated by B-70 which was correlated to astaxanthin ester biosynthesis. Nitrogen starvation, various LEDs and the identified upregulated genes may provide useful tools for future metabolic engineering to significantly increase free astaxanthin, its esters and fatty acid precursors in H. pluvialis.
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