期刊
BIORESOURCE TECHNOLOGY
卷 247, 期 -, 页码 940-946出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.09.187
关键词
Lactobacillus brevis; L-arabinose isomerase; D-tagatose; Spore surface-display; Immobilization
资金
- High-level talents project of Six Talent Peaks in Jiangsu Province [SWYY-018]
- [31571806]
- [31100045]
- [31270114]
In the present study, a new strain of Lactobacillus brevis producing D-tagatose was isolated and identified. Then, the L-arabinose isomerase (L-AI) of this strain was displayed on the spore surface of Bacillus subtilis DB403 by using an anchoring protein CotG and a peptide linker (Gly-Gly-Gly-Gly-Ser). This displayed L-AI with high specific activity and stability was used as a novel immobilized biocatalyst for producing D-tagatose through batch and semi-continuous biotransformation. The conversion rate of D-tagatose from 125 g/L D-galactose was achieved 79.7% at 28 h, and the volumetric productivity reached 4.3 g/L/h at 20 h. Furthermore, the displayed L-AI showed a good performance on the reusability and remained 87% of the specific activity and 40.7% of the conversion rate after five recycles. A high efficient immobilized method for producing food-grade D-tagatose was established using spore surface-displayed L-AI.
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