期刊
BIORESOURCE TECHNOLOGY
卷 260, 期 -, 页码 9-15出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.03.070
关键词
alpha, omega-Dodecanedioic acid; Hydrolysate of Candida cells; Candida viswanathii; alpha, omega-Dicarboxylic acids; Membrane bioreactor
资金
- National High Technology Research and Development Program of China [2015AA0210022015AA021002]
- National Science Foundation of China [21406240]
The aim of the present study is to develop an effective production process for a, omega-dodecanedioic acid (DC12) biosynthesis using n-dodecane and hydrolysate of Candida cells as substrates by membrane integrated repeated batch fermentation. Cells and n-dodecane were simultaneously recycled during the filtration of fermentation broth (FB) with a 150 kDa ceramic membrane under a cross-flow velocity of 4 m/s and a trans-membrane pressure of 0.2 MPa, and it was also revealed that the cells in the broth could alleviate the membrane fouling during the FB filtration. Moreover, the hydrolysate of the collected cells could be successfully used as a nitrogen source to replace 50% yeast extract for decreasing the DC12 production cost. With repeated-batch culture in a membrane bioreactor, the maximal DC12 productivity could be enhanced by 57.8% compared with the batch culture, meanwhile n-dodecane and cells could be recovered and used for the next fermentation cycle.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据