期刊
BIOPROCESS AND BIOSYSTEMS ENGINEERING
卷 41, 期 5, 页码 603-611出版社
SPRINGER
DOI: 10.1007/s00449-018-1895-2
关键词
Recombinase polymerase amplification; Lateral flow dipstick; Nucleic acid test; Salmonella; Shellfish
资金
- Ningbo Innovation Team [2015C110018]
- Ningbo Science and Technology Research Projects [2017C110003]
- Zhejiang Provincial Public Welfare Technology Program of China [2017C33133]
- K.C. Wang Magna Fund in Ningbo University (SS)
- Scientific Research Foundation of Graduate School of Ningbo University [G16091]
- Earmarked Fund for Modern Agro-industry Technology Research System, China [CARS-49]
- Zhejiang Xinmiao Talents Program [2015R405013]
Salmonella is a major pathogen that causes acute foodborne outbreaks worldwide. Seafood, particularly shellfish, is a proven source of Salmonella spp. infection because many people prefer to eat it raw or lightly cooked. However, traditional identification methods are too time-consuming and complex to detect contamination of bacteria in the food chain in a timely manner, and few studies have aimed to identify Salmonella in shellfish early in the supply chain. We herein developed a method for rapid detection of Salmonella in shellfish based on the method of recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD), which targets the invasion gene A (invA). The RPA-LFD was able to function at 30-45 degrees C, and at the temperature of 40 degrees C, it only took 8 min of amplification to reach the test threshold of amplicons. The established method had both a good specificity and a sensitivity of 100 fg DNA per reaction (20 mu L). Regarding practical performance, RPA-LFD performed better than real-time PCR. Another advantage of RPA-LFD is that it was capable of being performed without expensive equipments. Thus, RPA-LFD has potential for further development as a detection kit for Salmonella in shellfish and other foods under field conditions.
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