期刊
WORLD JOURNAL OF HEPATOLOGY
卷 8, 期 6, 页码 322-330出版社
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.4254/wjh.v8.i6.322
关键词
Burn injury; Morphology; Histophatology; Liver; Ciclooxigenase-2
资金
- Sao Paulo Research Foundation, FAPESP [11/22034-9]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [11/22034-9] Funding Source: FAPESP
AIM: To investigate effects of severe burn injury (BI) in rat liver through the histopathological and inflammatory markers analysis. METHODS: Forty-two male Wistar rats were distributed into two groups, control (C) and subjected to scald BI (SBI). The animals were euthanized one, four and 14 d post sham or 45% of the total body surface BI. Liver fragments were submitted to histopathological, morphoquantitative (hepatocyte area and cell density), ciclooxigenase-2 (COX-2) immunoexpression, and gene expression [real-time polymerase chain reaction for tumor necrosis factor (TNF)-alpha, inducible nitric oxide synthase (iNOS) and caspase-3] methods. RESULTS: Histopathological findings showed inflammatory process in all periods investigated and hepatocyte degeneration added to increased amount of connective tissue 14 d post injury. Hepatocyte area, the density of binucleated hepatocytes and density of sinusoidal cells of SBI groups were increased when compared with control. COX-2 immunoexpression was stronger in SBI groups. No differences were found in TNF-alpha, iNOS and caspase-3 gene expression. CONCLUSION: BI induces histopathological changes, upregulation of COX-2 immunoexpression, and cell proliferation in liver of rats.
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