期刊
FERTILITY AND STERILITY
卷 104, 期 2, 页码 312-+出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2015.05.003
关键词
MicroRNA; spermatozoa; miR-34c; ICSI outcome; pregnancy
资金
- Zhejiang Province Key Science and Technology Innovation Team Independent Application Project [2011R50013-22]
- National Basic Research Program of People's Republic of China [2012CB944902]
- Natural Science Foundation [81170567, 81370761]
Objective: To assess the effects of micro ribonucleic acid (miR)-34b/c expression levels in human spermatozoa on intracytoplasmic sperm injection (ICSI) outcomes. Design: Retrospective observational study. Setting: In vitro fertilization center. Patient(s): A total of 162 patients with idiopathic male infertility who had undergone first ICSI cycles. Intervention(s): None. Main Outcome Measure(s): The levels of miR-34b/c in spermatozoa were measured using real-time polymerase chain reaction. Fertilization, early cleavage, day-3 good-quality embryo, pregnancy, implantation, and live birth rate were assessed. A receiver operating characteristic curve was employed to analyze the cutoff values. Result(s): No correlation was found between the spermatozoa miR-34b/c levels and the 2 pronuclei early cleavage rate. A correlation was seen between an increased level of miR-34c and a higher percentage of good-quality embryos on day 3. Although miR-34b and miR-34c levels were higher in the pregnancy group, compared with the nonpregnancy group, receiver operating characteristic curve analysis showed that miR-34c levels in spermatozoa were more strongly correlated with ICSI treatment outcomes, compared with miR-34b (area under the curve = 0.75). Patients in the miR-34c-positive group were more likely to exhibit higher rates of good-quality embryos, implantation, pregnancy, and live birth. A multivariable logistic regression analysis showed that miR-34c in spermatozoa (odds ratio: 5.699, with 95% confidence interval [CI]: 2.687-12.088) and woman's age (odds ratio: 0.843, with 95% CI: 0.736-0.966) were the 2 parameters that were significantly correlated with pregnancy. Conclusion(s): Our results demonstrate that miR-34c levels in spermatozoa are correlated with ICSI outcomes, suggesting that paternal miR-34c may play a role in the early phases of embryonic development. Levels of MiR-34c in human spermatozoa may be used as an indicator for ICSI outcomes. (C) 2015 by American Society for Reproductive Medicine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据