期刊
BIOMOLECULAR NMR ASSIGNMENTS
卷 12, 期 2, 页码 221-224出版社
SPRINGER
DOI: 10.1007/s12104-018-9812-9
关键词
Mitosis; Kinetochore; TACC3; ChTOG; Cell cycle
资金
- CRUK programme Grant [C24461/A12772]
- BBSRC [BB/L023113/1]
- BBSRC [BB/L023113/2, BB/L023113/1] Funding Source: UKRI
- MRC [MC_U117533887] Funding Source: UKRI
The microtubule regulatory protein colonic and hepatic tumor overexpressed gene (chTOG), also known as cytoskeleleton associated protein 5 (CKAP5) plays an important role in organizing the cytoskeleton and in particular in the assembly of k-fibres in mitosis. Recently, we dissected the hitherto poorly understood C-terminus of this protein by discovering two new domainsa cryptic TOG domain (TOG6) and a smaller, helical domain at the very C-terminus. It was shown that the C-terminal domain is important for the interaction with the TACC domain in TACC3 during the assembly of k-fibres in a ternary complex that also includes clathrin. Here we now present the solution NMR assignment of the chTOG C-terminal domain which confirms our earlier prediction that it is mainly made of -helices. However, the appearance of the H-1-N-15 HSQC spectrum is indicative of the presence of a considerable amount of unstructured and possibly flexible portions of protein in the domain.
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