期刊
BIOMEDICINE & PHARMACOTHERAPY
卷 105, 期 -, 页码 606-615出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2018.06.019
关键词
Solanine A; Solanum nigrum Linn.; Anti-inflammatory activity; NF-kappa B and MAPKs cascades; PI3K/Akt and JAK/STATs signalings
资金
- Health and Family Planning Commission of Sichuan Province [17PJ485]
- West Light Foundation of the Chinese Academy of Sciences [Y7C1031100]
Solanine A is a novel steroidal alkaloid isolated from Solarium nigrum Linn., a medicinal and edible plant which is widely used for treating various inflammatory diseases. In this study, we found that solanine A markedly suppressed the production of nitric oxide (NO) and prostaglandin E-2 (PGE(2)) in lipopolysaccharide/interferon-gamma (LPS/IFN gamma)-stimulated RAW264.7 cells, and attenuated xylene, carrageenan and agar-induced inflammation in mice. The mRNA levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX2), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and -1 beta (IL-1 beta), as well as C-X-C motif chemokine ligand-9 (CXCL9), were significantly decreased by solanine A. Furthermore, solanine A also suppressed LPS/IFN gamma-induced protein expression of iNOS and COX2. Mechanistically, solanine A inhibited the nuclear translocation of nuclear factor-kappa B (NF-kappa B) through the prevention of NF-kappa B p65 and inhibitory kappa B-alpha (I kappa B alpha) phosphorylation and I kappa B alpha degradation, and it also suppressed activation of extracellular regulated protein kinases (ERK), signal transducers and activators of transcription-1 (STAT1) and serine/threonine protein kinase Akt in LPS/IFN gamma-stimulated RAW264.7 macrophages and agar-induced granuloma model in mice. Taken together, solanine A exhibits a potent antiinflammatory activity in LPS/IFN gamma- activated macrophages and animal models of inflammation through inhibition of NF-kappa B, ERK1/2, Akt and STAT1 signaling pathways, suggesting that solanine A may be a valuable leading compound in the treatment of inflammatory diseases.
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