期刊
BIOMATERIALS
卷 180, 期 -, 页码 130-142出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2018.07.013
关键词
Collagen gel; Fibronectin; Vascular tissue engineering; Elastic fibers; Smooth muscle cells; Mechanical properties
资金
- NSERC CREATE Program in Regenerative Medicine
- Fonds de Recherche du Quebec - Nature et Technologies (FWO-Quebec)
- Natural Sciences and Engineering Research Council of Canada
- Heart and Stroke Foundation of Canada [HSFC G-16-00014634]
- Quebec Network for Oral and Bone Health Research
- CHU de Quebec
- Canada Research Chair program
One of the tightest bottlenecks in vascular tissue engineering (vTE) is the lack of strength and elasticity of engineered vascular wall models caused by limited elastic fiber deposition. In this study, flat and tubular collagen gel-based scaffolds were cellularised with vascular smooth muscle cells (SMCs) and supplemented with human plasma fibronectin (FN), a known master organizer of several extracellular matrix (ECM) fiber systems. The consequences of FN on construct maturation was investigated in terms of geometrical contraction, viscoelastic mechanical properties and deposition of core elastic fiber proteins. FN was retained in the constructs and promoted deposition of elastin by SMCs as well as of several proteins required for elastogenesis such as fibrillin-1, lysyl oxidase, fibulin-4 and latent TGF-beta binding protein-4. Notably, gel contraction, tensile equilibrium elastic modulus and elasticity were strongly improved in tubular engineered tissues, approaching the behaviour of native arteries. In conclusion, this study demonstrates that FN exerts pivotal roles in directing SMC-mediated remodeling of scaffolds toward the production of a physiological-like, elastin-containing ECM with excellent mechanical properties. The developed FN-supplemented systems are promising for tissue engineering applications where the generation of mature elastic tissue is desired and represent valuable advanced in vitro models to investigate elastogenesis. (C) 2018 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据