期刊
BIOMATERIALS
卷 178, 期 -, 页码 496-503出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2018.03.060
关键词
Hydrogel; Adaptable networks; Thioester exchange; Viscoelastic; Tissue engineering; Thiol-ene
资金
- NSF DMR [1408955]
- NIH NIDCR [DE016523]
- NSF GFRP
- NIH/CU Biophysics training program [T32 GM-065103]
- Arnold and Mabel Beckman Postdoctoral Fellowship
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [R01DE016523] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM065103] Funding Source: NIH RePORTER
The extracellular matrix (ECM) constitutes a viscoelastic environment for cells. A growing body of evidence suggests that the behavior of cells cultured in naturally-derived or synthetic ECM mimics is influenced by the viscoelastic properties of these substrates. Adaptable crosslinking strategies provide a means to capture the viscoelasticity found in native soft tissues. In this work, we present a covalent adaptable hydrogel based on thioester exchange as a biomaterial for the in vitro culture of human mesenchymal stem cells. Through control of pH, gel stoichiometry, and crosslinker structure, viscoelastic properties in these crosslinked networks can be modulated across several orders of magnitude. We also propose a strategy to alter these properties in existing networks by the photo-uncaging of the catalyst 4-mercaptophenylacetic acid. Mesenchymal stem cells encapsulated in thioester hydrogels are able to elongate in 3D and display increased proliferation relative to those in static networks. (C) 2018 Elsevier Ltd. All rights reserved.
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