期刊
BIOMATERIALS
卷 167, 期 -, 页码 44-57出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2018.03.024
关键词
TiO2 nanotubes; Oxidative stress; ITG alpha 5 beta 1; Wnt signals; Osteogenesis
资金
- State Key Project of Research and Development [2016YFC1100300, 2017YFB0702603]
- National Natural Science Foundation of China [51673032, 21734002, 31700827]
- Natural Science Foundation of Chongqing Municipal Government [CXTDX201601002]
- China Postdoctoral Science Foundation Grant [2017M622971]
- Innovation Team in University of Chongqing Municipal Government [CXTDX201601002]
- Fundamental Research Funds for the Central Universities [106112017CDJQJ238813, 10611CDJXZ238826]
Oxidative stress is commonly existed in bone degenerative disease (osteoarthritis, osteoporosis etc.) and some antioxidants had great potential to enhance osteogenesis. In this study, we aim to investigate the anti-oxidative properties of various TiO2 nanotubes (TNTs) so to screen the desirable size for improved osteogenesis and reveal the underlying molecular mechanism in vitro. Comparing cellular behaviors under normal and oxidative stress conditions, an interesting conclusion was obtained. In normal microenvironment, small TNTs were beneficial for adhesion and proliferation of osteoblasts, but large TNTs greatly increased osteogenic differentiation. However, after H2O2 (300 mu M) treatment (mimicking oxidative stress), only large TNTs samples demonstrated superior cellular behaviors of increased osteoblasts' adhesion, survival and differentiation when comparing with those of native titanium (control). Molecular results revealed that oxidative stress resistance of large nanotubes was closely related to the high expression of integrin alpha 5 beta 1 (ITG alpha 5 beta 1), which further up-regulated the production of anti-apoptotic proteins (p-FAK, p-Akt, p-FoxO3a and Bcl2) and down-regulated the expression of pro-apoptotic protein (Bax). Moreover, we found that Wnt signals (Wnt3a, Wnt5a, Lrp5, Lrp6 and beta-catenin) played an important role in promoting osteogenic differentiation of osteoblasts under oxidative condition. (C) 2018 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据