期刊
BIOFOULING
卷 34, 期 3, 页码 320-334出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2018.1437910
关键词
Reserpine; Pseudomonas aeruginosa; anti-biofilm; antivirulence; qRT-PCR; molecular docking
资金
- University of Kalyani
- Bose Institute
- University Grants Commission, India
This study aimed to evaluate the effect of reserpine, a plant-derived indole-alkaloid, against Pseudomonas aeruginosa PAO1 biofilms. The anti-biofilm activity of reserpine was evaluated by crystal violet staining, MTT assay, confocal laser scanning microscopy and scanning electron microscopy. Reserpine effects were also assessed by qRT-PCR of quorum sensing (QS)-regulated genes and biochemical quantification of the QS-mediated virulence factors pyocyanin, rhamnolipids, proteases and elastases. Reserpine reduced biofilm formation, cell motility, virulence factor production, and QS-controlled gene expression. Additionally, molecular docking analysis for AHL synthase LasI and QS transcriptional regulators LasR/MvfR revealed a plausible molecular mechanisms of reserpine QS inhibition. These findings provide insights into the underlying mode of action of reserpine, which may be useful in the development of new drugs against biofilm-related infections.
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