期刊
BIOELECTROCHEMISTRY
卷 120, 期 -, 页码 76-82出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2017.11.010
关键词
AC electrokinetics; Dielectrophoresis; Electrodes; Electroosmosis; Proteins
资金
- European Regional Development Fund (ERDF)
- Brandenburg Ministry of Sciences, Research and Cultural Affairs (MWFK) [85004117]
- German Research Foundation (DFG) [SPP 1857 HO1298/4-1]
Employing electric phenomena for the spatial manipulation of bioparticles from whole cells down to dissolved molecules has become a useful tool in biotechnology and analytics. AC electrokinetic effects like dielectrophoresis and AC electroosmosis are increasingly used to concentrate, separate and immobilize DNA and proteins. With the advance of photolithographical micro- and nanofabrication methods, novel or improved bioanalytical applications benefit from concentrating analytes, signal enhancement and locally controlled immobilization by AC electroldnetic effects. In this review of AC electrokinetics of proteins, the respective studies are classified according to their different electrode geometries: individual electrode pairs, interdigitated electrodes, quadrupole electrodes, and 3D configurations of electrode arrays. Known advantages and disadvantages of each layout are discussed. (C) 2017 Elsevier B.V. All rights reserved.
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