期刊
BIOELECTROCHEMISTRY
卷 119, 期 -, 页码 196-202出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2017.10.005
关键词
Extracellular electron transfer; Microbial fuel cells; Flavin; Electron shuttle; Bacillus megaterium
资金
- National Natural Science Foundation of China [51478451, 51208490, 21322703]
- Knowledge Innovation Program of the Chinese Academy of Sciences [IUEQN201306]
- Carlsberg Foundation [CF15-0164]
The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of strain LLD-1 was confirmed by electrochemical E-t and amperometric I-t tests. Flavins in culture suspension from strain LLD-1 were further proved to be able to act as electron shuttles, strengthening the electron transfer from LID-1 to the electrode. The output voltage and current output were increased 2.8 times and 3.7 times, respectively, by adding 100 nM exogenetic fiavins into microbial fuel cells inoculated with LLD-1. Electricity generation by LLD-1 from different carbon sources can be enhanced by adding 100 nM exogenetic fiavins. This study indicated that fiavins were essential to the EET process of the Gram-positive strain LID-1. Furthermore, a putative EET model for B. megaterium strain LID-1 and even for Gram-positive bacteria was proposed. (C) 2017 Elsevier B.V. All rights reserved.
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