期刊
BIOCONTROL SCIENCE AND TECHNOLOGY
卷 28, 期 2, 页码 142-156出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09583157.2018.1430743
关键词
Antifungal extracts; phytopathogens; sclerotia; Trichoderma species
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil)
- Fundacao Araucaria (Parana, Brazil)
Sclerotinia sclerotiorum is an important plant pathogen with worldwide distribution that causes severe economic losses of various agricultural crops such as soybean. This fungus is normally controlled with synthetic chemical fungicides that pose risks to the environment, and can be harmful to human health, and they can also induce resistance in pests. The aim of this study was to investigate the potential of Trichoderma asperelloides as a biocontrol agent towards white mold disease on soybeans crops. The antagonism of two strains of T. asperelloides (T25 and T42) isolated from soil samples was determined in-vitro by dual-culture confrontation testing on nine S.sclerotiorum strains obtained from sclerotia collected on diseased soybean plants. The mycelial growth and inhibition of carpogenic and ascospore germination by T.asperelloides extracts, as well as the efficacy of these on white mold control in soybeans were evaluated. Both strains of T.asperelloides exhibited high potential of antagonism. Methanolic and ethyl acetate extracts of the two T.asperelloides strains showed excellent growth inhibition (60-100%) on all of the pathogens tested. The ethyl acetate extracts of both T.asperelloides strains exhibited the highest efficacy against carpogenic germination, decreasing by 20-30% the number of ascospores per apothecium. Strains of T.asperelloides tested were more efficient in controlling white mold than two commercial products made from Trichoderma harzianum. The new strains of T.asperelloides have potential for successful biological control of white mold disease of soybean crops in the field.
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