期刊
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
卷 1866, 期 2, 页码 327-347出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2017.11.005
关键词
NAD(P)H-dependent oxidoreductases; Metabolic engineering; Protein engineering; Directed evolution; Enzyme promiscuity; Substrate specificity
资金
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/N503873/1]
- Syngenta [BB/N503873/1]
- BBSRC [BB/M011321/1, BB/M002454/1]
- Biotechnology and Biological Sciences Research Council [BB/M002454/1, BB/M011321/1] Funding Source: researchfish
- BBSRC [BB/M002454/1, 1658622, BB/M011321/1, BB/N503873/1] Funding Source: UKRI
NAD(P)H-dependent oxidoreductases catalyze the reduction or oxidation of a substrate coupled to the oxidation or reduction, respectively, of a nicotinamide adenine dinucleotide cofactor NAD(P)H or NAD(P). NAD(P)Hdependent oxidoreductases catalyze a large variety of reactions and play a pivotal role in many central metabolic pathways. Due to the high activity, regiospecificity and stereospecificity with which they catalyze redox reactions, they have been used as key components in a wide range of applications, including substrate utilization, the synthesis of chemicals, biodegradation and detoxification. There is great interest in tailoring NAD(P)H-dependent oxidoreductases to make them more suitable for particular applications. Here, we review the main properties and classes of NAD(P)H-dependent oxidoreductases, the types of reactions they catalyze, some of the main protein engineering techniques used to modify their properties and some interesting examples of their modification and application.
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