期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1862, 期 10, 页码 2271-2280出版社
ELSEVIER
DOI: 10.1016/j.bbagen.2018.07.015
关键词
ASK1; Oxidative stress; Apoptosis; beta-TrCP; E3 ubiquitin ligase; Ubiquitin-proteasome system
资金
- Japan Society for the Promotion of Science (JSPS) [JP25221302, JP26111007, JP16K18872, JP26114009]
- Kowa Life Science Foundation [2016 A-2]
Apoptosis signal-regulating kinase 1 (ASK1) is a key player in the homeostatic response of many organisms. Of the many functions of ASK1, it is most well-known for its ability to induce canonical caspase 3-dependent apoptosis through the MAPK pathways in response to reactive oxygen species (ROS). As ASK1 is a regulator of apoptosis, its proper regulation is critical for the well-being of an organism. To date, several E3 ubiquitin ligases have been identified that are capable of degrading ASK1, signifying the importance of maintaining ASK1 expression levels during stress responses. ASK1 protein regulation under unstimulated conditions, however, is still largely unknown. Using tandem mass spectrometry, we have identified beta-transducin repeat containing protein (beta-TrCP), an E3 ubiquitin ligase, as a novel interacting partner of ASK1 that is capable of ubiquitinating and subsequently degrading ASK1 through the ubiquitin-proteasome system (UPS). This interaction requires the seven WD domains of beta-TrCP and the C-terminus of ASK1. By silencing the beta-TrCP genes, we observed a significant increase in caspase 3 activity in response to oxidative stress, which could subsequently be suppressed by silencing ASK1. These findings suggest that beta-TrCP is capable of suppressing oxidative stress-induced caspase 3 dependent apoptosis through suppression of ASK1, assisting in the organism's ability to maintain homeostasis in an unstable environment.
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