期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1860, 期 2, 页码 624-632出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2017.11.005
关键词
Aerosol-OT; Bicontinuous microemulsions; Melittin; Antimicrobial peptides; Small-angle neutron scattering; Winsor-III microemulsion systems; Biomembrane mimetic systems
资金
- Laboratory Directed Research and Development program of Oak Ridge National Laboratory (ORNL) [6552]
- Office of Biological and Environmental Research of the U.S. Department of Energy (DOE)
- Scientific User Facilities Division, Office of Basic Energy Sciences, DOE
Antimicrobial peptides effectively kill antibiotic-resistant bacteria by forming pores in prokaryotes' biomembranes via penetration into the biomembranes' interior. Bicontinuous microemulsions, consisting of interdispersed oil and water nanodomains separated by flexible surfactant monolayers, are potentially valuable for hosting membrane-associated peptides and proteins due to their thermodynamic stability, optical transparency, low viscosity, and high interfacial area. Here, we show that bicontinuous microemulsions formed by negatively-charged surfactants are a robust biomembrane mimetic system for the antimicrobial peptide melittin. When encapsulated in bicontinuous microemulsions formed using three-phase (Winsor-III) systems, melittin's helicity increases greatly due to penetration into the surfactant monolayers, mimicking its behavior in biomembranes. But, the threshold melittin concentration required to achieve these trends is lower for the microemulsions. The extent of penetration was decreased when the interfacial fluidity of the microemulsions was increased. These results suggest the utility of bicontinuous microemulsions for isolation, purification, delivery, and host systems for antimicrobial peptides.
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