期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1861, 期 1, 页码 306-315出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2018.06.008
关键词
CER[EOS]; CER[AP]; CER[NP]; Neutron diffraction; LPP; Long periodicity phase
资金
- Deutsche Forschungsgemeinschaft [DFG NE427/30-1, DO 463/6-1]
- Evonik Industries AG
This study used neutron diffraction to investigate a ceramide-[NP] C24/[AP] C24/[EOS]-br C30/cholesterol/lignoceric acid (0.6: 0.3: 0.1: 0.7: 1) based stratum comeum modelling system. By adding specifically deuterated ceramides-[NP]-D-3, [AP] -D-3, and [EOS]-br-D-3, detailed information on the lamellar and the nanostructure of the system was obtained. For the short periodicity phase a natural-like lamellar repeat distance of 5.47 +/- 0.02 nm was observed, similar to the [NP]/[AP] base system without the [EOS]-br. Unlike in this system the ceramides here were slightly tilted, hinting towards a slightly less natural arrangement. Due to the deuteration it was possible to observe that the long ceramide chains were overlapping in the lamellar mid-plane. This is considered to be an important feature for the natural stratum corneum. Despite the presence of a ceramide [EOS] analogue-able to form a long phase arrangement - no distinct long periodicity phase was formed, despite a slightly higher than natural omega-acyl ceramide ratio of 10 mol%. The deuterated variant of this ceramide determined that the very long ceramide was integrated into the short periodicity phase, spanning multiple layers instead, The - compared to the base system - unchanged repeat distance highlights the stability of this structure. Furthermore, the localisation of the very long ceramide in the short periodicity phase indicates the possibility of a crosslinking effect and thus a multilayer stabilizing role for the ceramide [EOS]. It can be concluded, that additionally to the mere presence of ceramide-[EOS] more complex conditions have to be met in order to form this long phase. This has to be further investigated in the future.
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