Cytotoxic ergostane derivatives from the edible mushroom Termitomyces microcarpus (Lyophyllaceae)

Five ergostanes, dimethylincisterol (1), 5 alpha,8 alpha epidioxy-(22E,24R)-ergosta-6,9(11),22-trien-3 beta-ol (2), 5 alpha,8 alpha epidioxy-(22E,24R)-ergosta-6,22-dien-3 beta-ol (3), 5 alpha.,6 alpha-epoxy-(22E,24R)-ergosta-8(14),22-diene-3 beta,7 alpha-diol (4), (22E,24R)-ergosta-7,22-diene-3 beta,5 alpha,66-trio] (5) and betulinic acid (6) were isolated from the fresh fruiting bodies of the edible mushroom, Termitomyces microcarpus. The cytotoxicity of the compounds isolated was evaluated against the NCI 60 human cancer cell line panel. The degraded sterol dimethylincisterol (1) and 5 alpha,8 alpha epidioxyergosta-6,22-dien-3 beta-ol (3) possessed cytotoxic activity at the mu M level. (C) 2017 Elsevier Ltd. All rights reserved.