期刊
BIOCHEMICAL JOURNAL
卷 475, 期 -, 页码 1473-1489出版社
PORTLAND PRESS LTD
DOI: 10.1042/BCJ20170910
关键词
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资金
- Alberta Innovates Bio Solutions
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2014-04585, RGPIN-2016-05926]
- Canada Research Chairs Program
- VEGA agency grant [2/0180/12]
- Research & Development Operational Programs - ERDF [TRANSMED ITMS 26240120043, TRANSMED 2 ITMS 26240120044]
- China Scholarship Council
- Alberta Innovates Graduate Student Top-up Award
Seed oil from flax (Linum usitatissimum) is enriched in a-linolenic acid (ALA; 18:3 Delta(9cis, 12cis, 15cis)), but the biochemical processes underlying the enrichment of flax seed oil with this polyunsaturated fatty acid are not fully elucidated. Here, a potential process involving the catalytic actions of long-chain acyl-CoA synthetase (LACS) and diacylglycerol acyltransferase (DGAT) is proposed for ALA enrichment in triacylglycerol (TAG). LACS catalyzes the ATP-dependent activation of free fatty acid to form acyl-CoA, which in turn may serve as an acyl-donor in the DGAT-catalyzed reaction leading to TAG. To test this hypothesis, flax LACS and DGAT cDNAs were functionally expressed in Saccharomyces cerevisiae strains to probe their possible involvement in the enrichment of TAG with ALA. Among the identified flax LACSs, LuLACS8A exhibited significantly enhanced specificity for ALA over oleic acid (18:1 Delta(9cis)) or linoleic acid (18:2 Delta(9cis, 12cis)). Enhanced alpha-linolenoyl-CoA specificity was also observed in the enzymatic assay of flax DGAT2 (LuDGAT2-3), which displayed similar to 20 times increased preference toward a-linolenoyl-CoA over oleoyl-CoA. Moreover, when LuLACS8A and LuDGAT2-3 were co-expressed in yeast, both in vitro and in vivo experiments indicated that the ALA-containing TAG enrichment process was operative between LuLACS8A- and LuDGAT2-3-catalyzed reactions. Overall, the results support the hypothesis that the cooperation between the reactions catalyzed by LACS8 and DGAT2 may represent a route to enrich ALA production in the flax seed oil.
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