期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 130, 期 -, 页码 10-20出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2017.11.004
关键词
Umbilicaria esculenta; Polysaccharide; Adsorption; Biosorption; Separation; Purification
资金
- Natural Science Foundation of Jiangsu Province [BK20171379]
- Jiangsu Science and Technology Supporting Special Item for Northern Jiangsu [BN2015071]
- Enterprise Graduate Workstation of Nanjing Agriculture University
- Enterprise Graduate Workstation of Jiangsu Province (Nanjing Agriculture University)
- Enterprise Graduate Workstation of Jiangsu Province (Jiangsu Lianyi Biological Technology Co., Ltd.)
- Enterprise Graduate Workstation of Jiangsu Province (Nantong Chitsuru Foods Co., Ltd.)
Umbilicaria esculenta as an edible macrolichen possesses nutritional properties, such as antioxidant, immune-boosting, and lipid peroxidation inhibitory properties. A new enrichment and separation technology for the polysaccharides from Umbilicaria esculenta (UEP) with AB-8 resin was established, and its adsorption data were well fitted to the pseudo-second-order kinetic model and the Lang-muir isotherm, with a process of monolayer coverage of UEP onto it. After purification by DEAE (diethylaminoethyl) Cellulose-52 chromatography, two polysaccharide fractions (UEP-1, UEP-2) were obtained and characterized. UEP-1 was composed of mannose, rhamnose, glucose and galactose in a molar ratio of 0.59:3.12:93.09:3.20 with a molecular weight of 124.7 kD, while UEP-2 comprised man nose and glucose in a molar ratio of 20.66:7934 with a molecular weight of 249.4 kD. With DPPH (2, 2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate), superoxide anion, and hydroxyl scavenging capacities as indices, their antioxidative assays in vitro indicated that the polysaccharides exhibited the very strong scavenging activities in a concentration-dependent manner. It was worth expecting that UEP could be developed as a novel potential functional component in food industries. (C) 2017 Elsevier B.V. All rights reserved.
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