期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 503, 期 4, 页码 2386-2392出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2018.06.166
关键词
F plasmid; T4SS; Conjugation; TrbC; TraW; Protein-protein interactions
资金
- Natural Sciences & Engineering Council of Canada (NSERC) [RGPIN-06218-2014]
- Canada Foundation for Innovation (CFI) [12939]
- York University
Bacterial conjugation, such as that mediated by the E. coli F plasmid, is a main mechanism driving bacterial evolution. Two important proteins required for F-pilus assembly and DNA transfer proficiency are TraW and TrbC. As members of a larger complex, these proteins assemble into a type IV secretion system and are essential components of pore formation and mating pair stabilization between the donor and the recipient cells. In the current report, we demonstrate the physical interaction of TraW and TrbC, show that TraW preferentially interacts with the N-terminal domain of TrbC, and that this interaction is important in restoring conjugation in traW/trbC knockouts. (C) 2018 Elsevier Inc. All rights reserved.
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