期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 502, 期 2, 页码 202-208出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2018.05.144
关键词
Delavatine A; Anti-inflammation; Cytokine; NO; NF-kappa B; Microglia
资金
- National Key Research and Development Program of China [2017YFC1702000]
- NSFC [81573318, 81230090, 81520108030, 81373301, 1302658]
- Shanghai Engineering Research Center for the Preparation of Bioactive Natural Products [10DZ2251300]
- Scientific Foundation of Shanghai China [12401900801, 13401900101]
- National Major Project of China [2011ZX09307-002-03]
- National Key Technology R&D Program of China [2012BAI29B06]
- State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University) [CMEMR2016-B]
Delavatine A, an unusual isoquinoline alkaloid isolated from I. delavayi, was first studied for antiinflammatory effect using lipopolysaccharide (LPS)-induced BV-2 microglia. In the present study, we found that delavatine A substantially suppressed the LPS-induced pro-inflammatory mediators, nitric oxide (NO), and tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), interleukin-1 beta (1-1 beta) in BV-2 microglial cells. These effects resulted from the inhibition of their regulatory genes inducible NO synthase (iNOS), cycloxygenase-2 (COX-2) and TNF-alpha, IL-6, IL-beta. In addition, we examined several pathways related to inflammation. The results revealed that delavatine A significantly decreased LPS-induced the activation of nuclear factor-kappa B (NF-kappa B) by suppressing the p65 subunits, and the phosphorylation of IKBot, while not related to PI3K/Akt or MAPK pathways. (C) 2018 Elsevier Inc. All rights reserved.
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