期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 499, 期 3, 页码 642-647出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2018.03.203
关键词
Tenocyte; Biomimetic hydrogel; Cell adhesion peptide; Stiffness; Mechanotransduction; Cyclic tensile strain
资金
- National Institutes of Health [1R21AR062197, R01 HL119371]
- Arthritis Research UK studentship [NE/PhD/19598]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL119371] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R21AR062197] Funding Source: NIH RePORTER
We recently developed a fiber composite consisting of tenocytes seeded onto discontinuous fibers embedded within a hydrogel, designed to mimic physiological tendon micromechanics of tension and shear. This study examined if cell adhesion peptide (DGEA or YRGDS), fiber modulus (50 or 1300 kPa) and/or cyclic strain (5% strain, 1 Hz) influenced bovine tenocyte gene expression. Ten genes were analyzed and none were sensitive to peptide or fiber modulus in the absence of cyclic tensile strain. Genes associated with tendon (SOX and TNMD), collagens (COL1A1, COL3A1, COL11A1), and matrix remodelling (MMPI, MMP2, and TIMP3) were insensitive to cyclic strain. Contrarily, cyclic strain up regulated 1L6 by 30-fold and MMP3 by 10-fold in soft YRGDS fibers. IL6 expression in soft YRGDS fibers was 5.7 and 3.3-fold greater than in soft DGEA fibers and stiff RGD fibers, respectively, under cyclic strain. Our findings suggest that changes in the surrounding matrix can influence catabolic genes in tenocytes when cultured in a complex strain environment mimicking that of tendon, while having minimal effects on tendon and homeostatic genes. (C) 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).
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