期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 495, 期 1, 页码 1028-1033出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2017.11.115
关键词
Astrocyte co-culture; Human induced-pluripotent stem cell-derived neurons; Synchronization; Multi-electrode array
资金
- Alpha Med Scientific
- XCell Science Company
- AMED [17bk0104076h0201]
- Kaken-hi [17K20111, 17H05939, 17H05551]
- Konica Minolta Science and Technology Foundation
- Grants-in-Aid for Scientific Research [15H05569, 17K20111, 17H05551, 17H05939, 17K15577, 16K14554, 16J02472] Funding Source: KAKEN
In culture conditions, human induced-pluripotent stem cells (hiPSC)-derived neurons form synaptic connections with other cells and establish neuronal networks, which are expected to be an in vitro model system for drug discovery screening and toxicity testing. While early studies demonstrated effects of co-culture of hiPSC-derived neurons with astroglial cells on survival and maturation of hiPSC-derived neurons, the population spiking patterns of such hiPSC-derived neurons have not been fully characterized. In this study, we analyzed temporal spiking patterns of hiPSC-derived neurons recorded by a multi-electrode array system. We discovered that specific sets of hiPSC-derived neurons co-cultured with astrocytes showed more frequent and highly coherent non-random synchronized spike trains and more dynamic changes in overall spike patterns over time. These temporally coordinated spiking patterns are physiological signs of organized circuits of hiPSC-derived neurons and suggest benefits of co-culture of hiPSC-derived neurons with astrocytes. (C) 2017 Elsevier Inc. All rights reserved.
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