期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 17, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/ijms17020083
关键词
sesquiterpenes; leukemia cells; cell cycle; apoptosis
资金
- Aktion Osterreich-Ungarn Universitatslehrerstipendien and Apaczai Csere Janos Doctoral Fellowship [A2-ACSJD-12]
- New Hungary Development Plan [TAMOP-4.2.4 A/2-11-1-2012-0001]
- Hungarian Scientific Research Fund [OTKA K109293]
Plants and plant extracts play a crucial role in the research into novel antineoplastic agents. Four sesquiterpene lactones, artecanin (1), 3-chloro-4,10-dihydroxy-1,2-epoxy-5,7H-guaia-11(13)-en-12,6-olide (2), iso-seco-tanapartholide 3-O-methyl ether (3) and 4,15-dihydro-3-dehydrozaluzanin C (4), were isolated from two traditionally used Asteraceae species (Onopordum acanthium and Artemisia asiatica). When tested for antiproliferative action on HL-60 leukemia cells, these compounds exhibited reasonable IC50 values in the range 3.6-13.5 M. Treatment with the tested compounds resulted in a cell cycle disturbance characterized by increases in the G1 and G2/M populations, while there was a decrease in the S phase. Additionally, 1-3 elicited increases in the hypodiploid (subG1) population. The compounds elicited concentration-dependent chromatin condensation and disruption of the membrane integrity, as revealed by Hoechst 33258-propidium staining. Treatment for 24 h resulted in significant increases in activity of caspases-3 and -9, indicating that the tested sesquiterpenes induced the mitochondrial pathway of apoptosis. The proapoptotic properties of the sesquiterpene lactones were additionally demonstrated withannexin V staining. Compounds 1 and 2 increased the Bax/Bcl-2 expression and decreased the expressions of CDK1 and cyclin B2, as determined at the mRNA level by means of RT-PCR. These experimental results indicate that sesquiterpene lactones may be regarded as potential starting structures for the development of novel anticancer agents.
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