期刊
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
卷 5, 期 -, 页码 186-192出版社
ELSEVIER
DOI: 10.1016/j.bcab.2016.01.008
关键词
Metagenomic library; Cellulase; Function based screening; beta-1, 4-endoglucanase; Paper and pulp mill
资金
- Post Graduate School, Indian Agricultural Research Institute (New Delhi, India)
- NAIP [IARI 70-24]
- ICAR NFBSFARA
A metagenomic library consisting of 7500 clones was constructed from the soil continuously irrigated with effluents of paper and pulp mill. A cellulase positive clone H-10 was identified by function based screening. A beta-1, 4- endoglucanase gene with an open reading frame of 1499 bp was identified from this positive clone. The deduced amino acid sequence analysis revealed that amplified beta-1, 4-endoglucanase belongs to GH5 and shares 98% similarity with Bacillus subtilis (EF070194), 95% with Bacillus subtilis IARISP-1(KF240847) 96% with B. subtilis (AY183475) and 95% with uncultured bacterium clone (JX567739). Clone H-10 indicated higher CMCase activity of 1 IU/ml on the substrate CMC as compared to rice straw (0.5 IU/ml). The characterization of enzymes revealed its pH optima between 8.5-9 and retained 80% of activity at pH 11. The thermal profile of the enzyme indicated broad range of thermotolerance. (C) 2016 Elsevier Ltd. All rights reserved.
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