期刊
FEBS JOURNAL
卷 282, 期 16, 页码 3060-3074出版社
WILEY
DOI: 10.1111/febs.13212
关键词
dehydrogenase; enzyme design; oxidase; oxyanion hole; oxygen reactivity
资金
- FWF ('Fonds zur Forderung der wissenschaftlichen Forschung') through SFB ('Spezialforschungsbereich') projects [F1805, F4604]
- PhD program 'Molecular Enzymology' [W901]
- European Molecular Biology Organization
- Institute of Molecular Biosciences, University of Graz, Austria
- BBSRC [BB/L000121/1] Funding Source: UKRI
- Austrian Science Fund (FWF) [W 901] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/L000121/1] Funding Source: researchfish
The ability of flavoenzymes to reduce dioxygen varies greatly, and is controlled by the protein environment, which may cause either a rapid reaction (oxidases) or a sluggish reaction (dehydrogenases). Previously, a gatekeeper' amino acid residue was identified that controls the reactivity to dioxygen in proteins from the vanillyl alcohol oxidase superfamily of flavoenzymes. We have identified an alternative gatekeeper residue that similarly controls dioxygen reactivity in the grass pollen allergen Phlp4, a member of this superfamily that has glucose dehydrogenase activity and the highest redox potential measured in a flavoenzyme. A substitution at the alternative gatekeeper site (I153V) transformed the enzyme into an efficient oxidase by increasing dioxygen reactivity by a factor of 60000. An inverse exchange (V169I) in the structurally related berberine bridge enzyme (BBE) decreased its dioxygen reactivity by a factor of 500. Structural and biochemical characterization of these and additional variants showed that our model enzymes possess a cavity that binds an anion and resembles the oxyanion hole' in the proximity of the flavin ring. We showed also that steric control of access to this site is the most important parameter affecting dioxygen reactivity in BBE-like enzymes. Analysis of flavin-dependent oxidases from other superfamilies revealed similar structural features, suggesting that dioxygen reactivity may be governed by a common mechanistic principle. DatabaseStructural data are available in PDB database under the accession numbers , , , , , and .
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