期刊
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
卷 38, 期 7, 页码 E117-E134出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/ATVBAHA.118.310934
关键词
endothelial cells; regeneration; stem cells; substance P; tissue engineering
资金
- National Nature Science Foundation of China [81530059, 81772000]
- KIST Institutional Program
- KU-KIST Graduate School of Converging Science and Technology Program
Objective The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell-derived factor)-1-derived peptide and SP (substance P) for in situ vascular regeneration. Approach and Results Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1-derived peptide were fabricated by electrospinning. SP and SDF-1 peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1 and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1 grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood vessel formation, which was the most prominent in the SP grafts. Evaluation of inflammatory response showed that 3 groups did not significantly differ in terms of the numbers of proinflammatory macrophages, whereas SP grafts showed significantly higher numbers of proremodeling macrophages than that of the control and SDF-1 grafts. Conclusions SDF-1 and SP grafts can be potential candidates for in situ vascular regeneration and are worthy for future investigations.
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