期刊
AQUACULTURE
卷 495, 期 -, 页码 106-114出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2018.05.038
关键词
Acute stress; Crowding; Liver; Proteomics; Recovery
资金
- Khorramshahr University of Marine Science and Technology [145]
Proteomics has been used to analyze the global alterations in fish protein abundance in response to various kinds of stressors, but proteomics surveys concerning the post-stress recovery have not been performed. As liver is the main organ in the homeostatic adjustments to stress, we used a proteomics approach to address molecular response in this tissue during post-stress recovery in rainbow trout. The stressor consisted of a crowding (200 kg fishm-3, 45 min) by decreasing water volume in tanks. Blood samples were obtained prior to and 0, 2, 4, 8 and 24 h after stress. The acute stress response and recovery was confirmed by the transient changes in serum metabolites, which returned to pre-stress levels over a 24 h period. The liver tissues were also collected prior to (control group) and 24 h after stress (recovery group) for proteomics study. A total of 20 spots, which relative amount changed at least 2-fold under the applied conditions, were successfully identified by MALDI-TOF/TOF MS. The spots found to increase in the recovery group were identified as follow: adenosylhomocysteinase, glyceraldehyde 3-phosphate dehydrogenase, fructose-bisphosphate aldolase, 2-peptidylprolyl isomerase, fatty acid-binding protein, and serum albumin. Nine proteins including malate dehydrogenase, serum albumin, nucleoside diphosphate kinase, 2-peptidylprolyl isomerase, hydroxyprostaglandin dehydrogenase, peroxiredoxin, ATP synthase subunit d, glycine-rich RNA-binding protein, and transketolase-like protein were found to decrease in the recovery group. Our results suggest that the recovery after stress cause a switch from aerobic energy production toward anaerobic energy production in the liver cells of recovering fish.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据