期刊
AQUACULTURE
卷 482, 期 -, 页码 103-110出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquaculture.2017.09.030
关键词
Ammonia; Biofloc; Illumina-MiSeq; Microbial diversity; Ammonia-oxidizing bacteria
资金
- earmarked fund for China Agriculture Research System [CARS-45]
- State Science and Technology Support Program of China [2012BAD25B06]
- Agro-scientific Research in the Public Interest Project [201203083]
- Fundamental Research Funds for Innovation Center of Hubei Province [2016ZXPY02]
The main objective of this work was to investigate how different carbon sources affect microbial diversity of biofloc. The experiment consisted of the control and three types of biofloc systems using different carbon sources, tapioca starch (TS), plant cellulose (PC) and the combination of tapioca starch and plant cellulose (TS + PC), and conducted for 42 days in twelve 300 L Fiber Reinforce Plastic (FRP) circular tanks. Pelteobagrus vachelli (n = 72) of mean weight (38.1 +/- 5.9 g) were randomly distributed into twelve tanks. Community characterization of total bacteria and ammonia-oxidizing bacteria in all treatments were respectively investigated by Illumina MiSeq analysis using PCR-amplified 16S rRNA gene fragments (primer 27F-338R for total bacteria and primer CTO189F-CTO654R for ammonia-oxidizing bacteria). The ammonia-nitrogen concentration in control (3.6 +/- 4.6 mg L-1) was significantly higher than that in all biofloc systems (2.4 +/- 2.9 mg L-1, 1.8 +/- 2.4 mg L-1 and 2.2 +/- 2.5 mg L-1 for TS, PC and TS + PC, respectively) (P < 0.05). Weight gain ratio in TS was significantly higher than the control (P < 0.05), while there is no significant difference between the control and treatment groups added plant cellulose (PC and TS + PC) (P > 0.05). The Illumina MiSeq sequencing results showed that biofloc systems incorporating plant cellulose treatment groups had a higher total bacterial diversity and greater microbial richness than those with no plant cellulose treatment groups (Control and TS) (P < 0.05). There was significant increase of Betaproteobacteria in the control and TS treatments, whereas Alphaproteobacteria was higher in treatment groups added plant cellulose (P < 0.05). Illumina sequencing analysis of ammonia-oxidizing bacteria V3 region also detected a higher community diversity in biofloc systems (Shannon index,> 1.21; Simpson index,< 0.3949) compared to control (Shannon index, 0.84; Simpson index, 0.6149). We conclude that organic carbon sources in biofloc system can decrease ammonia-nitrogen concentration and improve the community diversities of overall and ammonia-oxidizing bacteria.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据