期刊
FEMS MICROBIOLOGY ECOLOGY
卷 92, 期 5, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsec/fiw059
关键词
branched-chain fatty acids; Illumina MiSeq; volatile fatty acids
类别
资金
- Agriculture and Food Research Initiative Competitive Grant from the United States Department of Agriculture National Institute of Food and Agriculture [2014-67016-21791]
- United States Department of Agriculture Hatch Grant [VT-H01801]
- University of Vermont Dairy Center of Excellence
- NIFA [688186, 2014-67016-21791] Funding Source: Federal RePORTER
Rumen bacteria form a dynamic, complex, symbiotic relationship with their host, degrading forages to provide volatile fatty acids (VFA) and other substrates as energy to the animal. The objectives were to characterize rumen bacteria in three genetic lines of primiparous dairy cattle, Holstein (HO, n = 7), Jersey (JE, n = 8), and HO x JE crossbreeds (CB, n = 7) across a lactation [3, 93, 183 and 273 days in milk (DIM)] and correlate these factors with VFA, bacterial cell membrane fatty acids (FA), and animal production (i. e. milk yield). This study employed Illumina MiSeq (v. 3) to investigate rumen bacterial communities and gas-liquid chromatography/mass spectroscopy to identify bacterial membrane FA. Lactation stage had a prominent effect on rumen bacterial communities, whereas genetics had a lesser effect on rumen bacteria. The FA composition of bacterial cell membranes was affected by both lactation stage and genetics. Few correlations existed between VFA and bacterial communities; however, moderate correlations occurred between milk yield, protein percentage, fat yield and rumen bacterial communities. Positive correlations were found between branched-chain FA (BCFA) in bacterial cell membranes and bacterial genera. In conclusion, bacterial communities and their FA compositions are more affected by stage of lactation than by genetics of dairy cow.
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