期刊
APPLIED PHYSIOLOGY NUTRITION AND METABOLISM
卷 43, 期 12, 页码 1257-1267出版社
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
DOI: 10.1139/apnm-2017-0629
关键词
insulin secretion; Ca2+ oscillation; protein kinase A; protein kinase C; nutritional recovery
资金
- CAPES [PROCAD - AUXPE 708/2008]
- CNPq [620106/2008-5]
- FAPEMAT [512642/2009]
We investigated the insulin release induced by glucose, the Ca2+ oscillatory pattern, and the cyclic AMP (cAMP)/protein kinase A (PKA) and phospholipase C (PLC)/protein kinase C (PKC) pathways in islets from adult rats that were reared under diets with 17% protein (C) or 6% protein (LP) during gestation, suckling, and after weaning and in rats receiving diets with 6% protein during gestation and 17% protein after birth (R). First-phase glucose-induced insulin secretion was reduced in LP and R islets, and the second phase was partially restored in the R group. Glucose stimulation did not modify intracellular Ca2+ concentration, but it reduced the Ca2+ oscillatory frequency in the R group compared with the C group. Intracellular cAMP concentration was higher and PKA-C alpha expression was lower in the R and LP groups compared with the C group. The PKC alpha content in islets from R rats was lower than that in C and LP rats. Thus, nutritional recovery from a low-protein diet during fetal life did not repair the kinetics of insulin release, impaired Ca2+ handling, and altered the cAMP/PKA and PLC/PKC pathways.
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