期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 102, 期 19, 页码 8403-8417出版社
SPRINGER
DOI: 10.1007/s00253-018-9205-0
关键词
TGEV S protein; Lactobacillus plantarum; Oral immunization; DCpep; Bcells; Immune response
资金
- National Key Research and Development Program of China [2017YFD0501000, 2017YFD0501200]
- National Natural Science Foundation of China [31672528, 31602092]
- Science and Technology Development Program of Jilin Province [20160519011JH, 20170204034NY, 20180520037JH]
- Special Funds for Industrial Innovation of Jilin Province [2016C063]
- Thirteen Five-year Plan for Sci & Tech Research Program of Jilin Education Department of P.R. China [JJKH20170318KJ]
- Scientific Research Foundation of Jilin Agricultural University of China [201601]
Transmissible gastroenteritis coronavirus (TGEV) is one of the most severe threats to the swine industry. In this study, we constructed a suite of recombinant Lactobacillus plantarum with surface displaying the spike (S) protein coming from TGEV and fused with DC cells targeting peptides (DCpep) to develop an effective, safe, and convenient vaccine against transmissible gastroenteritis. Our research results found that the recombinant Lactobacillus plantarum (NC8-pSIP409-pgsA-S-DCpep) group expressing S fused with DCpep could not only significantly increase the percentages of MHC-II(+)CD80(+) B cells and CD3(+)CD4(+) T cells but also the number of IgA(+) B cells and CD3(+)CD4(+) T cells of ileum lamina propria, which elevated the specific secretory immunoglobulin A (SIgA) titers in feces and IgG titers in serum. Taken together, these results suggest that NC8-pSIP409-pgsA-S-DCpep expressing the S of TGEV fused with DCpep could effectively induce immune responses and provide a feasible original strategy and approach for the design of TGEV vaccines.
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