期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 17, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/ijms17060986
关键词
polysaccharide; Morchella esculenta; chemical structure; anti-proliferating activity; pulsed electric field
资金
- Jilin Province Natural Science Foundation Fellowship [20140101036JC]
- Jilin Agriculture Science and Technology College, Food Science and Engineering Key Subject foundation fellowship [2015X061]
Polysaccharides from Morchella esculenta have been proven to be functional and helpful for humans. The purpose of this study was to investigate the chemical structure and anti-proliferating and antitumor activities of a Morchella esculenta polysaccharide (MEP) extracted by pulsed electric field (PEF) in submerged fermentation. The endo-polysaccharide was separated and purified by column chromatography and Gel permeation chromatography, and analyzed by gas chromatography. The MEP with an average molecular weight of 81,835 Da consisted of xylose, glucose, mannose, rhamnose and galactose at the ratio of 5.4:5.0:6.5:7.8:72.3. Structure of MEP was further analyzed by Fourier-transform infrared spectroscopy and H-1 and C-13 liquid-state nuclear magnetic resonance spectroscopy. Apoptosis tests proved that MEP could inhibit the proliferation and growth of human colon cancer HT-29 cells in a time-and dose-dependent manner within 48 h. This study provides more information on chemical structure of anti-proliferating polysaccharides isolated from Morchella esculenta.
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