期刊
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
卷 54, 期 4, 页码 396-403出版社
PLEIADES PUBLISHING INC
DOI: 10.1134/S0003683818040154
关键词
Trichoderma pseudokoningii; trichokonins; Phalaenopsis; Botrytis cinerea; inhibition; apoptosis
资金
- Nature Science Fund of Shandong province [ZR2013CM006]
- Science and Technology Program of Shandong province [2014GNC110020]
- Technology Innovation Program of Liaocheng University [2013A01]
- Shandong Provincial Key Laboratory of Agricultural Microbiology Open Fund [SDKL2017015]
Trichokonins (TKs) are antimicrobial peptaibols extracted from Trichoderma pseudokoningii strain SMF2. In this paper, it was discovered that TK VI, the main active ingredient of TKs, had a profound inhibitory effect on the growth and sporulation of the moth orchid gray mold, Botrytis cinerea. In addition, TK VI increased the cell membrane permeability of the pathogen. Further investigation of nuclear DNA fragmentation, subcellular structure disintegration, and mitochondrial membrane potential depolarization, as well as the appearance of reactive oxygen species, indicated that TK VI could induce programmed cell death in the necrotrophic pathogenic fungus B. cinerea.
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