期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 187, 期 1, 页码 338-351出版社
HUMANA PRESS INC
DOI: 10.1007/s12010-018-2824-1
关键词
High-ammonia-nitrogen wastewater; Hydrogen-oxidizing bacterium; Nitrogen assimilation; Single-cell protein; Mixotrophy; Paracoccus
资金
- National Key R&D Program of China [2018YFD0501405]
- Youth Innovation Promotion Association CAS [2017423]
- Key Project for Foreign Cooperation of the International Cooperation Bureau of the Chinese Academy of Sciences [182344KYSB20170009]
- Science and Technology Service Network Initiative (STS) of the Chinese Academy of Sciences
- Key Laboratory of Environmental and Applied Microbiology of Chengdu Institute of Biology CAS [KLCAS-2016-10, KLCAS-2017-9]
- Chengdu Science and Technology Huimin Project [2016-HM02-00092-SF]
To recover a nitrogen resource from high-ammonia-nitrogen wastewater, two amphitrophic hydrogen-oxidizing bacteria (HOB), Paracoccus denitrificans Y5 and P. versutus D6, capable of nitrogen assimilation for single-cell protein (SCP) production were isolated. These two HOB strains could grow autotrophically with H-2 as an electron donor, O-2 as an electron acceptor, CO2 as a carbon source, and ammonia nitrogen (NH4+-N) as a nitrogen source. The cell molecular formulas of strains Y5 and D6 determined by autotrophic cultivation were C3.33H6.83O2.58N0.77 and C2.87H5.34O3.17N0.57, respectively. The isolated strains could synchronously remove NH4+-N and organic carbon and produce SCP via heterotrophic cultivation. The rates of removal of NH4+-N and soluble chemical oxygen demand reached 35.47 and 49.04%, respectively, for Y5 under mixotrophic cultivation conditions with biogas slurry as a substrate. SCP content of strains Y5 and D6 was 67.34-73.73% based on cell dry weight. Compared with soybean meal, the SCP of Y5 contained a variety of amino acids.
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