期刊
BIOMEDICAL MATERIALS
卷 11, 期 3, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1748-6041/11/3/035019
关键词
asymmetric membrane; nanofibers; tissue engineering
资金
- National Natural Science Foundation of China [NNSFC 31570964, 2013 B090200007, 20114401110003, JCYJ20130401174221011]
- Marine Economy Innovation and Development Project [GD2012-D01-002]
- Marine Fishery Science and Technology Promotion Project of Guangdong [A1201301C06]
- Marine Strategic Emerging Industry Special Project [GD2013-B02-003, JCYJ20140905153253218]
Asymmetric membranes, which mimic the structure and functions of human skin, have been extensively pursued as ideal skin tissue engineering constructs. In this study, we demonstrated that nanostructured asymmetric membranes can be prepared by the self-organization of chemically heterogeneous bilayer electrospun membranes in aqueous solutions. Structurally, the skin layer consists of hydrophobic beta-glucan butyrate nanofibers and its inner layer consists of hydrophilic beta-glucan acetate nanofibers. After the electrospinning process, both of the layers are in a dense state. When placed in water, the skin layer absorbs little water and still remains dense, but the fibers in the inner layer become extensively hydrated and spontaneously reorganize into a fully stretched structure, resulting in a significant volume increase and a density decrease of the inner layer. SEM imaging showed that beta-glucan ester nanofibers exhibited a bead-free and uniform structure. Contact angle measurement and swelling tests showed that the inner layer was highly hydrophilic with extensive swelling, but the skin layer was highly hydrophobic with little swelling. Mechanical tests indicated that the nanofibrous asymmetric membranes had good mechanical properties in both the dry and wet states. In vitro cytocompatibility tests showed that nanofibrous asymmetric membranes could promote the adhesion and proliferation of fibroblasts and keratinocytes. A preliminary in vivo study performed on a full thickness mouse skin wound model demonstrated that the nanofibrous asymmetric membranes significantly accelerated the wound healing process by promoting re-epithelialization, tissue remodeling and collagen deposition. Taken together, our study provides a novel model for the design and fabrication of nanostructured asymmetric membranes, and our beta-glucan based nanofibrous asymmetric membranes could be used as an advanced platform for skin tissue engineering.
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