Enhanced Efflux Pump Activity in Old Candida glabrata Cells

We investigated the effect of replicative aging on antifungal resistance in Candida glabrata. Our studies demonstrate significantly increased transcription of ABC transporters and efflux pump activity in old versus young C. glabrata cells of a fluconazole-sensitive and -resistant strain. In addition, higher tolerance to killing by micafungin and amphotericin B was noted and is associated with higher transcription of glucan synthase gene FKS1 and lower ergosterol content in older cells.