期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 62, 期 6, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00206-18
关键词
oxacillin resistance determinants; MRSA; guanine metabolism
资金
- US Public Health Service Award [2 R01 AI457838-15]
- FEDER funds through COMPETE-Programa Operacional Competitividade e Internacionalizacao (POCI) [LISBOA-01-0145-FEDER-007660]
- FCT-Fundacao para a Ciencia e a Tecnologia and project ONEIDA [LISBOA-01-0145-FEDER-016417]
- FEEI-Fundos Europeus Estruturais e de Investimento from Programa Operacional Regional Lisboa
- FCT [SFRH/BPD/111697/2015]
- PCORI [CER-1402-10800]
- RB Roberts Bacterial Antibiotic Resistance Group (BARG)
Methicillin-resistant Staphylococcus aureus (MRSA) strains carry either a mecA- or a mecC-mediated mechanism of resistance to beta-lactam antibiotics, and the phenotypic expression of resistance shows extensive strain-to-strain variation. In recent communications, we identified the genetic determinants associated with the stringent stress response that play a major role in the antibiotic resistant phenotype of the historically earliest archaic clone of MRSA and in the mecC-carrying MRSA strain LGA251. Here, we sought to test whether or not the same genetic determinants also contribute to the resistant phenotype of highly and homogeneously resistant (H*R) derivatives of a major contemporary MRSA clone, USA300. We found that the resistance phenotype was linked to six genes (fruB, gmk, hpt, purB, prsA, and relA), which were most frequently targeted among the analyzed 20 H*R strains (one mutation per clone in 19 of the 20 H*R strains). Besides the strong parallels with our previous findings (five of the six genes matched), all but one of the repeatedly targeted genes were found to be linked to guanine metabolism, pointing to the key role that this pathway plays in defining the level of antibiotic resistance independent of the clonal type of MRSA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据