期刊
ANTICANCER RESEARCH
卷 38, 期 6, 页码 3501-3505出版社
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.12621
关键词
Cystathionine; brain cancer cells; thiols; sulfurtransferases; hydrogen sulfide
类别
资金
- National Committee for Scientific Research [K/ZDS/005887]
Background/Aim: In certain cancers, accumulation of cystathionine has been observed. The present study investigated the effect of cystathionine on astrocytoma (U373) cell proliferation, the activity of gamma-cystathionase (CTH) and changes in thiols levels. Materials and Methods: The methods used in the study included cytotoxicity assay, crystal violet staining method, CTH activity assay and reverse phase-high performance liquid chromatography (RP-HPLC). Results: The addition of cystathionine to the culture medium resulted in an increase of cystathionine level in U373 cells after 24 h of culture. Reduction of intracellular cystathionine level after 48 and 72 h of culture was associated with increased L-cysteine and L-cystine levels and stimulation of cell proliferation. Interestingly, a decrease in intracellular L-cysteine and L-cystine levels during the first hours of culture was observed. Conclusion: Elevated levels of cystathionine resulted in increased U373 cell proliferation by increasing the L-cysteine levels and GSH/GSSG ratio (especially after 72 h of the culture), but not with a simultaneous increase in the levels of total glutathione.
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