期刊
ANNUAL REVIEW OF BIOPHYSICS, VOL 47
卷 47, 期 -, 页码 355-376出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev-biophys-070317-032905
关键词
CLARITY; hydrogels; metareactant; HTC; hydrogel-tissue; clearing
类别
资金
- NATIONAL INSTITUTE OF MENTAL HEALTH [R01MH086373] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [DP2NS087949] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG047664] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON DRUG ABUSE [P50DA042012, R37DA035377] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [OT2OD023848] Funding Source: NIH RePORTER
- Howard Hughes Medical Institute Funding Source: Medline
- NIA NIH HHS [R01 AG047664] Funding Source: Medline
- NIDA NIH HHS [R37 DA035377, P50 DA042012] Funding Source: Medline
- NIH HHS [OT2 OD023848] Funding Source: Medline
- NIMH NIH HHS [R01 MH086373, R01 MH075957] Funding Source: Medline
- NINDS NIH HHS [DP2 NS087949] Funding Source: Medline
Over the past five years, a rapidly developing experimental approach has enabled high-resolution and high-content information retrieval from intact multicellular animal (metazoan) systems. New chemical and physical forms are created in the hydrogel-tissue chemistry process, and the retention and retrieval of crucial phenotypic information regarding constituent cells and molecules (and their joint interrelationships) are thereby enabled. For example, rich data sets defining both single-cell-resolution gene expression and single-cell-resolution activity during behavior can now be collected while still preserving information on three-dimensional positioning and/or brain-wide wiring of those very same neurons-even within vertebrate brains. This new approach and its variants, as applied to neuroscience, are beginning to illuminate the fundamental cellular and chemical representations of sensation, cognition, and action. More generally, reimagining metazoans as metareactants-or positionally defined three-dimensional graphs of constituent chemicals made available for ongoing functionalization, transformation, and readout-is stimulating innovation across biology and medicine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据