期刊
ANNUAL REVIEW OF BIOPHYSICS, VOL 47
卷 47, 期 -, 页码 377-397出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev-biophys-070317-033239
关键词
G protein-coupled receptor; lipidic cubic phase; serial femtosecond crystallography; structure-function; X-ray free-electron laser
类别
资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM108635] Funding Source: NIH RePORTER
- NIGMS NIH HHS [R01 GM108635] Funding Source: Medline
G protein-coupled receptors (GPCRs) represent a large superfamily of membrane proteins that mediate cell signaling and regulate a variety of physiological processes in the human body. Structure-function studies of this superfamily were enabled a decade ago by multiple breakthroughs in technology that included receptor stabilization, crystallization in a membrane environment, and microcrystallography. The recent emergence of X-ray free-electron lasers (XFELs) has further accelerated structural studies of GPCRs and other challenging proteins by overcoming radiation damage and providing access to high-resolution structures and dynamics using micrometer-sized crystals. Here, we summarize key technology advancements and major milestones of GPCR research using XFELs and provide a brief outlook on future developments in the field.
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